Mitochondrial diseases result from failures of the mitochondria, specialized compartments present in every
cell of the body except red blood cells.
Mitochondria are responsible for creating more than 90% of the energy needed by the body to sustain life and
support growth. When they fail, less and less energy is generated within the cell. Cell injury and even cell death
follow. If this process is repeated throughout the body, whole systems begin to fail, and the life of the person
in whom this is happening is severely compromised. The disease primarily affects children, but adult onset is
becoming more and more common.
Diseases of the mitochondria appear to cause the most damage to cells of the brain, heart, liver, skeletal muscles,
kidney and the endocrine and respiratory systems.
Depending on which cells are affected, symptoms may include loss of motor control, muscle weakness and pain,
gastro-intestinal disorders and swallowing difficulties, poor growth, cardiac disease, liver disease, diabetes, respiratory
complications, seizures, visual/hearing problems, lactic acidosis, developmental delays and susceptibility to
Energy Factories and Much More
The conventional teaching in biology and medicine is that mitochondria function only as “energy factories” for the cell.
This over-simplification is a mistake which has slowed our progress toward understanding the biology underlying
It takes about 3000 genes to make a mitochondrion. Mitochondrial DNA encodes just 37 of these genes; the remaining
genes are encoded in the cell nucleus and the resultant proteins are transported to the mitochondria. Only about 3%
of the genes necessary to make a mitochondrion (100 of the 3000) are allocated for making ATP. More than 95%
(2900 of 3000) are involved with other functions tied to the specialized duties of the differentiated cell in which it resides.
These duties change as we develop from embryo to adult, and our tissues grow, mature, and adapt to the postnatal
environment. These other, non-ATP-related functions are intimately involved with most of the major metabolic pathways
used by a cell to build, break down, and recycle its molecular building blocks. Cells cannot even make the RNA and DNA
they need to grow and function without mitochondria.
The building blocks of RNA and DNA are purines and pyrimidines. Mitochondria contain the rate-limiting enzymes for
pyrimidine biosynthesis (dihydroorotate dehydrogenase) and heme synthesis (d-amino levulinic acid synthetase) required
to make hemoglobin. In the liver, mitochondria are specialized to detoxify ammonia in the urea cycle. Mitochondria are also
required for cholesterol metabolism, for estrogen and testosterone synthesis, for neurotransmitter metabolism, and for free
radical production and detoxification. They do all this in addition to breaking down (oxidizing) the fat, protein, and carbohydrates
we eat and drink.
Defining Mitochondrial Disease
Mitochondrial diseases are the result of either inherited or spontaneous mutations in mtDNA or nDNA which lead to altered
functions of the proteins or RNA molecules that normally reside in mitochondria. Problems with mitochondrial function,
however, may only affect certain tissues as a result of factors occurring during development and growth that we do not
yet understand. Even when tissue-specific isoforms of mitochondrial proteins are considered, it is difficult to explain the
variable patterns of affected organ systems in the mitochondrial disease syndromes seen clinically.
Genocopies of Mitochondrial Disease
Because mitochondria perform so many different functions in different tissues, there are literally hundreds of different
mitochondrial diseases. Each disorder produces a spectrum of abnormalities that can be confusing to both patients
and physicians in early stages of diagnosis. Because of the complex interplay between the hundreds of genes and cells
that must cooperate to keep our metabolic machinery running smoothly, it is a hallmark of mitochondrial diseases that
identical mtDNA mutations may not produce identical diseases. Genocopies are diseases that are caused by the same
mutation but which may not look the same clinically.
Phenocopies of Mitochondrial Disease
The converse is also true: different mutations in mtDNA and nDNA can lead to the same diseases. In genetics, these are
known as phenocopies. A good example is Leigh syndrome, which can be caused by about a dozen different gene defects.
Leigh syndrome, originally a neuropathological description of the brain of one affected child, was described by Denis Leigh,
the distinguished British physician, in 1951. It is characterized by bilaterally symmetrical MRI abnormalities in the brain
stem, cerebellum, and basal ganglia, and often accompanied by elevated lactic acid levels in the blood or cerebrospinal fluid.
Leigh syndrome may be caused by the NARP mutation, the MERRF mutation, complex I deficiency, cytochrome oxidase
(COX) deficiency, pyruvate dehydrogenase (PDH) deficiency, and other unmapped DNA changes. Not all children with these
DNA abnormalities will go on to develop Leigh syndrome, however.
Mitochondrial diseases are even more complex in adults because detectable changes in mtDNA occur as we age and, conversely,
the aging process itself may result from deteriorating mitochondrial function. There is a broad spectrum of metabolic, inherited
and acquired disorders in adults in which abnormal mitochondrial function has been postulated or demonstrated.